Service description:
mRNA are enriched from total RNA and fragmented mRNA are subsequently converted to cDNA for library preparation.
After that cDNA libraries are sequenced using Illumina PE150 bp sequencing strategy.
Sample requirement:
Total RNA ≥ 200ng
Concentration ≥ 10ng/μl
RIN ≥ 6.5
A260/280 = 1.8-2.2
mRNA sequencing is a powerful next-generation sequencing technique used to map and quantify messenger RNA transcripts in a cell.
Key Applications:
Gene Expression Profiling: Accurately measures levels of gene expression, letting researchers compare active genes between healthy and diseased tissues.
Discovery of Novel Transcripts: Identifies unknown gene isoforms, alternative splicing events, and rare mutations without relying on prior genomic annotations.
Detection of Gene Fusions: Pinpoints abnormal fusion genes common in various cancers and genetic disorders.
Allele-Specific Expression: Analyzes differential expression between maternal and paternal alleles to study imprinting and genetic variants.
Biomarker Research: Helps uncover disease-specific markers for diagnostics and therapeutic target selection.

Contact: Emily Tan
Phone: +65 80842349
E-mail: info@avenir-biotech.com
Whatsapp:+65 80842349
Add: Rooms1318-19,13/F,Hollywood Plaza, 610 Nathan Road, Mongkok, Kowloon, Hong Kong