microRNA Sequencing

MicroRNA sequencing is a powerful next-generation sequencing application used to profile, discover, and quantify small non-coding RNA molecules.

Key Applications:

• Novel miRNA Discovery: Identifies previously unknown microRNAs and rare variants across poorly characterized species or specific cell types.

• Biomarker Identification: Detects disease-specific expression signatures in biofluids (liquid biopsies) for non-invasive diagnostics and prognostics.

• Isoform (isomiR) Profiling: Measures sequence variations at the 3' or 5' ends of mature miRNAs to study alternative processing and biogenesis.

• Target Gene Integration: Pairs miRNA expression data with bulk mRNA sequencing to map negative correlations and validate post-transcriptional gene regulation.

• RNA Modification and Interaction Studies: Inspects nucleotide-level changes, RNA editing, and secondary structures to clarify RNA-protein interactions.