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Amplicon Sequencing

Service description:
Amplified PCR products submitted by customer are examined for quality and are subsequently ligated with Illumina adaptors (PCR-free) to be ready-for-sequencing libraries.
After that libraries are sequenced using various Illumina sequencing strategies such as PE150, PE250 and PE300 bp.

Sample requirement:
Amount ≥ 1μg
Concentration ≥ 50ng/μl
Volume ≥ 20 μL
A260/280 = 1.8-2.0

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Amplicon sequencing is a targeted sequencing method that uses polymerase chain reaction (PCR) to selectively amplify specific DNA fragments or genomic regions of interest. Specific primers are designed to flank the target region and the resulting amplicons are then sequenced to determine the nucleotide sequence.

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